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Differential regulation of macrophage ccaat- enhancer binding protein isoforms by lipopolysaccharide and cytokines

Tengku-Muhammad, Tengu S., Hughes, Timothy R., Ranki, Harri, Cryer, Anthony and Ramji, Dipak Purshottam 2000. Differential regulation of macrophage ccaat- enhancer binding protein isoforms by lipopolysaccharide and cytokines. Cytokine 12 (9) , pp. 1430-1436. 10.1006/cyto.2000.0711

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Abstract

The regulation of the C/EBP family in macrophages by LPS and cytokines is of potentially crucial importance in several pathophysiological conditions. The action of LPS and three cytokines on the expression of C/EBP mRNA, protein and functional DNA binding activity in the murine J774.2 cell line was therefore studied. Exposure of the cells to LPS, IL-1, IFN-γ and TNF-α produced a reduction of C/EBPα mRNA levels and a corresponding increase in the expression of C/EBPβ and C/EBPδ. EMSA showed time-dependent changes in the DNA binding activity of individual C/EBP isoforms and demonstrated the participation of heterodimers between the different members in DNA–protein interactions. Additionally, mediator-specific changes in the kinetics and magnitude of C/EBP mRNA expression pattern and profile of DNA-protein interactions were observed. These studies provide novel insights into the potential mechanisms that may be responsible for the mediator-specific regulation of macrophage gene expression through the C/EBP family.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: CCAAT-enhancer binding protein; cytokines; J774.2 cell line; lipopolysaccharide; gene expression.
Publisher: Elsevier
ISSN: 1043-4666
Last Modified: 26 Oct 2017 13:47
URI: http://orca.cf.ac.uk/id/eprint/63450

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