Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The ovine CCAAT-enhancer binding protein δ gene: cloning, characterization, and species-specific autoregulation

Davies, Gareth E., Sabatakos, Georgios, Cryer, Anthony and Ramji, Dipak Purshottam 2000. The ovine CCAAT-enhancer binding protein δ gene: cloning, characterization, and species-specific autoregulation. Biochemical and Biophysical Research Communications 271 (2) , pp. 346-352. 10.1006/bbrc.2000.2630

Full text not available from this repository.

Abstract

Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the regulation of gene expression during growth, differentiation, apoptosis, and inflammation. Autoregulation is relatively common in the modulation of C/EBP gene expression and, for the human and murine C/EBPα, it is known that species-specific autoregulatory mechanisms operate. It is therefore essential to investigate the autoregulation of additional C/EBP genes from a wider range of different species to gauge the degree of commonality, or otherwise, which exists. As an important step towards this goal, we report here the cloning and the characterisation of the ovine C/EBPδ gene (ovC/EBPδ) and analysis of its promoter region. Transient transfection assays reveal that ovC/EBPδ acts as a transcriptional activator. Although several motifs that are characteristic of C/EBPδ genes are conserved in the ovine sequence, including the basic region, leucine zipper, and activation domains, two regions have been identified that are specifically absent in the ovine and bovine homologues. The ovC/EBPδ promoter is active in both the hepatoma Hep3B and the mammary epithelial HC11 cell lines, induced by the cytokine interleukin-6 and autoregulated by mechanisms that are potentially different from those described for the rat promoter. These results suggest that, in common with C/EBPα, the C/EBPδ genes may also be subject to autoregulation by distinct species-specific mechanisms.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0006-291X
Last Modified: 04 Jun 2017 06:40
URI: http://orca.cf.ac.uk/id/eprint/63454

Citation Data

Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item