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Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-Carotene 15,15′-Monooxygenase

Culligan, Eamonn P., Sleator, Roy D., Marchesi, Julian Roberto and Hill, Colin 2014. Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-Carotene 15,15′-Monooxygenase. PLoS ONE 9 (7) , e103318. 10.1371/journal.pone.0103318

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Abstract

The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Systems Immunity Research Institute (SIURI)
Additional Information: © 2014 Culligan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher: Public Library of Science
ISSN: 1932-6203
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 29 June 2014
Last Modified: 21 Feb 2019 16:42
URI: http://orca.cf.ac.uk/id/eprint/63994

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Cited 10 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 1 time in Web of Science. View in Web of Science.

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