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Naturally-occurring and recombinant forms of the aspartic proteinases plasmepsins I and II from the human malaria parasite Plasmodium falciparum

Tyas, Lorraine, Gluzman, Ilya, Moon, Richard P., Rupp, Katharina, Westling, Jennifer, Ridley, Robert G., Kay, John, Goldberg, Daniel E. and Berry, Colin 1999. Naturally-occurring and recombinant forms of the aspartic proteinases plasmepsins I and II from the human malaria parasite Plasmodium falciparum. FEBS Letters 454 (3) , pp. 210-214. 10.1016/S0014-5793(99)00805-4

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Abstract

Comparable kinetic parameters were derived for the hydrolysis of peptide substrates and the interaction of synthetic inhibitors with recombinant and naturally-occurring forms of plasmepsin II. In contrast, recombinant plasmepsin I was extended by 12 residues at its N-terminus relative to its naturally-occurring counterpart and a 3–10-fold diminution in the kent values was measured for substrate hydrolysis by the recombinant protein. However, comparable K1 values were derived for the interaction of two distinct inhibitors with both forms of piasmepsin I, thereby validating the use of recombinant material for drug screening. The value of plasmepsin I inhibitors was determined by assessing their selectivity using human aspartic proteinases.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Malaria; Aspartic proteinase; Plasmepsin; Plasmodium falciparum.
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 04 Jun 2017 06:47
URI: http://orca.cf.ac.uk/id/eprint/64822

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