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Physicochemical and biological characterisation of single-walled and double-walled carbon nanotubes in biological media

Lui, Wen-T, Bien, Mauo-Ying, Chuang, Kai-Jen, Chang, Ta-Yuang, Jones, Timothy Peter, Berube, Kelly Ann, Lalev, Georgi, Dai-Hua, Tsai, Chuang, Hsiao-Chi and Chen, Tsun-Jen 2014. Physicochemical and biological characterisation of single-walled and double-walled carbon nanotubes in biological media. Journal of Hazardous Materials 280 , pp. 216-225. 10.1016/j.jhazmat.2014.07.069

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Abstract

To study the toxicity of nanoparticles under relevant conditions, it is important to reproducibly disperse nanoparticles in biological media in in vitro and in vivo studies. Here, single-walled nanotubes (SWNTs) and double-walled nanotubes (DWNTs) were physicochemically and biologically characterized when dispersed in phosphate-buffered saline (PBS) and bovine serum albumin (BSA). BSA-SWNT/DWNT interaction resulted in a reduction of aggregation and an increase in particle stabilization. Based on the protein sequence coverage and protein binding results, DWNTs exhibited higher protein binding than SWNTs. SWNT and DWNT suspensions in the presence of BSA increased interleukin-6 (IL-6) levels and reduced tumor necrosis factor-alpha (TNF-α) levels in A549 cells as compared to corresponding samples in the absence of BSA. We next determined the effects of SWNTs and DWNTs on pulmonary protein modification using bronchoalveolar lavage fluid (BALF) as a surrogate collected form BALB/c mice. The BALF proteins bound to SWNTs (13 proteins) and DWNTs (11 proteins), suggesting that these proteins were associated with blood coagulation pathways. Lastly, we demonstrated the importance of physicochemical and biological alterations of SWNTs and DWNTs when dispersed in biological media, since protein binding may result in the misinterpretation of in vitro results and the activation of protein-regulated biological responses.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QE Geology
Q Science > QP Physiology
Uncontrolled Keywords: Carbon nanotube; Inflammation; Nanomaterials; Nanotoxicology; Proteomics
Publisher: Elsevier
ISSN: 0304-3894
Date of Acceptance: 23 July 2014
Last Modified: 17 Jun 2019 02:51
URI: http://orca.cf.ac.uk/id/eprint/64967

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