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GABA and glycine-like immunoreactivity at axoaxonic synapses on 1a muscle afferent terminals in the spinal cord of the rat

Watson, Alan Hugh David and Bazzaz, Ayoub A. 2001. GABA and glycine-like immunoreactivity at axoaxonic synapses on 1a muscle afferent terminals in the spinal cord of the rat. The Journal of Comparative Neurology 433 (3) , pp. 335-348. 10.1002/cne.1143

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Abstract

The object of this study was to analyze the synaptic interactions of identified muscle spindle afferent axon terminals in the spinal cord of the rat. Group 1a muscle afferents supplying the gastrocnemius muscle were impaled with microelectrodes in the dorsal white matter of the spinal cord and stained by intracellular injection with Neurobiotin. Postembedding immunogold techniques were used to reveal GABA- and glycine-like immunoreactivity in boutons presynaptic to afferent terminals in the ventral horn and the deep layers of the dorsal horn. Serial-section reconstruction was used to reveal the distribution of synaptic contacts of different types on the afferent terminals. The majority of afferent boutons received axoaxonic and made axodendritic or axosomatic synaptic contacts. In the ventral horn, 91% of boutons presynaptic to the afferent terminals were immunoreactive for GABA alone and 9% were immunoreactive for both GABA and glycine. The mean number of axo-axonic contacts received per terminal was 2.7, and the mean number of synaptic contacts at which the terminal was the presynaptic element was 1.4. In the deep layers of the dorsal horn, 58% of boutons presynaptic to afferent terminals were immunoreactive for GABA alone, 31% were immunoreactive for GABA and glycine, and 11% for glycine alone. The mean number of axoaxonic contacts received per afferent terminal in this region was 1.6 and the mean number of synaptic contacts at which the terminal was the presynaptic element was 0.86. This clearly establishes the principle that activity in 1a afferents is modulated by several neurochemically distinct populations of presynaptic neuron.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley-Blackwell
ISSN: 0021-9967
Last Modified: 02 May 2019 11:33
URI: http://orca.cf.ac.uk/id/eprint/65027

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