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Extraction and sensitive detection of toxins A and B from the human pathogen clostridium difficile in 40 seconds using microwave-accelerated metal-enhanced fluorescence

Joshi, Lovleen Tina, Mali, Buddha L., Geddes, Chris D. and Baillie, Les 2014. Extraction and sensitive detection of toxins A and B from the human pathogen clostridium difficile in 40 seconds using microwave-accelerated metal-enhanced fluorescence. PLoS ONE 9 (8) , e104334. 10.1371/journal.pone.0104334

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Abstract

Clostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key step in reducing the devastating impact of this disease. The bacterium produces two toxins, A and B, which are thought to be responsible for the majority of the pathology associated with the disease, although the relative contribution of each is currently a subject of debate. For this reason we have developed a rapid detection assay based on microwave-accelerated metal-enhanced fluorescence which is capable of detecting the presence of 10 bacteria in unprocessed human feces within 40 seconds. These promising results suggest that this prototype biosensor has the potential to be developed into a rapid, point of care, real time diagnostic assay for C. difficile.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Additional Information: This PDF uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1932-6203/ [accessed 06/10/2014].
Publisher: Public Library of Science
ISSN: 1932-6203
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 10 July 2014
Last Modified: 23 Jan 2020 13:46
URI: http://orca.cf.ac.uk/id/eprint/65113

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