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A rapid determination of taurine in human plasma by LC

Ghandforoush-Sattari, Mohammadreza, Mashayekhi, Siminozar, Nemati, Mahboub and Routledge, Philip Alexander 2009. A rapid determination of taurine in human plasma by LC. Chromatographia 69 (11-12) , pp. 1427-1430. 10.1365/s10337-009-1055-6

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Abstract

Taurine is an amino acid which is not incorporated into proteins but found in the cytosol of many mammalian cells, in high concentrations (2–30 mM). Increase in plasma taurine concentration has already been reported after surgical trauma, X-radiation, muscle necrosis, carbon tetrachloride-induced liver damage, and paracetamol overdose. Plasma taurine concentration was measured using LC with fluorescence detection following derivatization by o-phtalaldehyde plus 3-mercapto-propionic acid and α-aminobutyric acid as internal standard. Under these conditions the retention time of taurine was 10 min. This method was sensitive enough, to quantify 150 pg mL−1 and detect 50 pg mL−1 of taurine ranging normally between 65 and 179 mmol L−1 (8–22 μg mL−1). The validated method allowed simple determination of human plasma taurine in pharmacokinetic and biomarker studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Column liquid chromatography; Taurine; α-Aminobutyric acid, o-phtalaldehyde, and 3-mercapto-propionic acid
Publisher: Springer Verlag
ISSN: 0009-5893
Last Modified: 04 Jun 2017 01:57
URI: http://orca.cf.ac.uk/id/eprint/6523

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