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Minisatellite DNA markers in the chicken genome. II isolation and characterization of minisatellite loci

Bruford, Michael William, Hanotte, O. and Burke, T. 1994. Minisatellite DNA markers in the chicken genome. II isolation and characterization of minisatellite loci. Animal Genetics 25 (6) , pp. 391-399.

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Abstract

The isolation of chicken minisatellites is reported. Two charomid libraries were constructed from fractions of size-selected MboI-digested genomic DNA. A total of 5985 colonies were screened with the multilocus probes 33.6 and 33.15; 137 positives were obtained (2.35%). A total of 55 clones were tested against four unrelated chickens; 30 revealed variable single locus patterns. More detailed characterization was made of 15 probes, which were tested against four families comprising a total of 53 offspring. Of parent/offspring comparisons 51% were informative for segregation. No mutations were detected. Three minisatellites were linked on the same autosome, implying that these loci may occur in clusters, and one was Z chromosome-linked. Heterozygosity and allelic variability were measured in 67 individuals from several different strains and breeds. Mean heterozygosity ranged from 50 to 84%. Minisatellite loci appear to be highly variable in the chicken and should provide highly informative markers in intraspecific crosses in genome mapping studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Subjects: Q Science > QH Natural history > QH426 Genetics
Publisher: Wiley-Blackwell
ISSN: 0268-9146
Last Modified: 04 Jun 2017 06:50
URI: http://orca.cf.ac.uk/id/eprint/65262

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