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Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells

Rinaldi, F., Hartfield, E. M., Crompton, L. A., Badger, J. L., Glover, C. P., Kelly, Claire, Rosser, Anne Elizabeth, Uney, J. B. and Caldwell, M. A. 2014. Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells. Cell Death and Disease 5 (1) , e1017. 10.1038/cddis.2013.546

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Abstract

Connexin43 (Cx43) is the most widely and abundantly expressed gap junction (GJ) protein and it is strongly associated with the regulation of cell cycle progression. Emerging roles for Cx43 in cell adhesion and migration during neural differentiation have also been recently recognized, and this has emphasized the involvement of Cx43 in different physiological process beyond its role as a GJ protein. In this study, we explore the function of Cx43 in the differentiation of human neural progenitor cells (hNPCs) using viral vectors that mediate the overexpression or knockdown of the protein. Results showed that in the absence of this protein fetal cortex-derived hNPCs differentiated toward a neuronal phenotype at expenses of a glial phenotype. Furthermore, the silencing of Cx43 did not affect hNPC proliferation rate or numbers of apoptotic cells. The increase in the number of neurons was not recapitulated when GJ intercellular communications were pharmacologically blocked, and this suggested that Cx43 was influencing hNPCs differentiation with a GJ-independent effect. In addition, Cx43 knockdown significantly increased β-catenin signaling, which has been shown to regulate the transcription of pro-neuronal genes during embryonic neural development. Our results add further support to the hypothesis that Cx43 protein itself regulates key signaling pathways during development and neurogenesis beyond its role as GJ protein.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Medicine
Neuroscience and Mental Health Research Institute (NMHRI)
Publisher: Macmillan
ISSN: 2041-4889
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 4 November 2013
Last Modified: 31 May 2019 09:01
URI: http://orca.cf.ac.uk/id/eprint/65549

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