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Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

Martin, S. A. M., Taggart, J. B., Seear, P, Bron, J. E., Talbot, R., Teale, A. J., Sweeney, Glen E., Hoyheim, B., Houlihan, D. F., Tocher, D. R., Zou, J. and Secombes, C. J. 2007. Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray. Physiological genomics 32 (1) , pp. 33-44. 10.1152/physiolgenomics.00064.2007

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Abstract

Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Physiological Society
ISSN: 1094-8341
Last Modified: 02 May 2019 10:58
URI: http://orca.cf.ac.uk/id/eprint/65576

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