Immunochemical characterization of proteins from mouse liver plasma membranes

Gurd, J. W., Evans, William Howard and Perkins, H. R. 1973. Immunochemical characterization of proteins from mouse liver plasma membranes. Biochemical Journal -London- 135 (4) , pp. 827-832.

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Abstract

1. Antiserum was prepared in rabbits against a purified mouse liver plasma-membrane fraction. 2. The antiserum was made to react with an (125)I-labelled alkaline-EDTA extract of the plasma membranes, and the immunoprecipitate analysed by polyacrylamide-gel electrophoresis. Seven proteins were immunoprecipitated and a single glycoprotein present in the alkaline-EDTA-soluble fraction was found to be a major component. 3. The alkaline-EDTA-soluble fraction was analysed by two-dimensional immunoelectrophoresis and this procedure indicated the presence of six antigenic components. 4. The plasma membranes were also extracted with 1% deoxycholate-1% Triton X-100; 50% of the protein, 80% of the alkaline phosphodiesterase activity and 30% of the 5'-nucleotidase activity were solubilized. 5. Two-dimensional immunoelectrophoresis of the deoxycholate-Triton X-100 extract indicated the presence of six antigens. 6. The relative distribution of the six antigens among the fractions obtained during the extraction procedure was examined immunoelectrophoretically to provide information on their disposition within the membrane.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Publisher:	Portland Press
ISSN:	0264-6021
Related URLs:	http://www.biochemj.org/bj/default.htm
Last Modified:	17 Mar 2021 02:51
URI:	https://orca.cardiff.ac.uk/id/eprint/65982

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