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The structural basis of differential inhibition of human calpain by indole and phenyl α--mercaptoacrylic acids

Adams, Sarah Elizabeth, Rizkallah, Pierre ORCID: https://orcid.org/0000-0002-9290-0369, Miller, David James, Robinson, Emma J., Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X and Allemann, Rudolf Konrad ORCID: https://orcid.org/0000-0002-1323-8830 2014. The structural basis of differential inhibition of human calpain by indole and phenyl α--mercaptoacrylic acids. Journal of Structural Biology 187 (3) , pp. 236-241. 10.1016/j.jsb.2014.07.004

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Abstract

Excessive activity of neutrophils has been linked to many pathological conditions, including rheumatoid arthritis, cancer and Alzheimer’s disease. Calpain-I is a Ca2+-dependent protease that plays a key role in the extravasation of neutrophils from the blood stream prior to causing damage within affected tissues. Inhibition of calpain-I with small molecule mercaptoacrylic acid derivatives slows the cell spreading process of live neutrophils and so these compounds represent promising drug leads. Here we present the 2.05 and 2.03 Å co-crystal X-ray structures of the pentaEF hand region, PEF(S), from human calpain with (Z)-3-(4-chlorophenyl)-2-mercaptoacrylic acid and (Z)-3-(5-bromoindol-3-yl)-2-mercaptoacrylic acid. In both structures, the α-mercaptoacrylic acid derivatives bind between two α-helices in a hydrophobic pocket that is also exploited by a leucine residue of the endogenous regulatory calpain inhibitor calpastatin. Hydrophobic interactions between the aromatic rings of both inhibitors and the aliphatic residues of the pocket are integral for tight binding. In the case of (Z)-3-(5-bromoindol-3-yl)-2-mercaptoacrylic acid, hydrogen bonds form between the mercaptoacrylic acid substituent lying outside the pocket and the protein and the carboxylate group is coplanar with the aromatic ring system. Multiple conformations of (Z)-3-(5-bromoindol-3-yl)-2-mercaptoacrylic acid were found within the pocket. The increased potency of (Z)-3-(5-bromoindol-3-yl)-2-mercaptoacrylic acid relative to (Z)-3-(4-chlorophenyl)-2-mercaptoacrylic acid may be a consequence of the indole group binding more deeply in the hydrophobic pocket of PEF(S) than the phenyl ring.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Medicine
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
Uncontrolled Keywords: Calpain-I; Mercaptoacrylic acids; PEF(S); Rheumatoid arthritis
Publisher: Elsevier
ISSN: 1047-8477
Date of First Compliant Deposit: 24 February 2017
Date of Acceptance: 24 July 2014
Last Modified: 05 May 2024 09:54
URI: https://orca.cardiff.ac.uk/id/eprint/66320

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