Nitrosylhemoglobin in photodynamically stressed human tumors growing in nude mice

Jakubowska, Monika, Michalczyk-Wetula, Dominika, Pyka, Janusz, Susz, Anna, Urbanska, Krystyna, Plonka, Beata K., Kuleta, Patryk, Lacki, Piotr, Krzykawska-Serda, Martyna, Fiedor, Leszek and Plonka, Przemyslaw M. 2013. Nitrosylhemoglobin in photodynamically stressed human tumors growing in nude mice. Nitric Oxide 35 , pp. 79-88. 10.1016/j.niox.2013.08.004

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Abstract

The role of nitric oxide in human tumor biology and therapy has been the subject of extensive studies. However, there is only limited knowledge about the mechanisms of NO production and its metabolism, and about the role NO can play in modern therapeutic procedures, such as photodynamic therapy. Here, for the first time, we report the presence of nitrosylhemoglobin, a stable complex of NO, in human lung adenocarcinoma A549 tumors growing in situ in nude mice. Using electron paramagnetic resonance spectroscopy we show that the level of nitrosylhemoglobin increases in the course of photodynamic therapy and that the phenomenon is local. Even the destruction of strongly vascularized normal liver tissue did not induce the paramagnetic signal, despite bringing about tissue necrosis. We conclude that photodynamic stress substantiates NO production and blood extravasation in situ, both processes on-going even in non-treated tumors, although at a lower intensity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Uncontrolled Keywords:	Adenocarcinoma; Electron paramagnetic resonance; Nitric oxide; Photodynamic therapy; Zinc pheophorbide a
Publisher:	Elsevier
ISSN:	1089-8603
Last Modified:	12 Jun 2019 03:00
URI:	https://orca.cardiff.ac.uk/id/eprint/66573

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