Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Intracellular prokaryotes in rumen ciliate protozoa: detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes

Lloyd, David, Williams, Alan G., Amann, Rudi, Hayes, Anthony Joseph, Durrant, Lisa and Ralphs, James Robert 1996. Intracellular prokaryotes in rumen ciliate protozoa: detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes. European Journal of Protistology 32 (4) , pp. 523-531. 10.1016/S0932-4739(96)80011-3

Full text not available from this repository.

Abstract

In situ hybridization of rumen ciliate protozoa with 16S ribosomal RNA fluorescent oligonucleotide probes specific for Archaea and Bacteria provided semi-quantitative indication of the location, type and extent of prokaryotic colonization of various protozoal species. The isotrichid holotrich ciliates generally carried a smaller load of intracellular microorganisms than did the entodiniomorphid species. Thus, the vast majority of the Dasytricha ruminantium population had neither bacterial not archaeal endosymbionts, although a very small minority of these ciliates (only 11 out of 447: < 3%), contained numerous Bacteria cells. Their food vacoules contained only Bacteria. Thirty per cent of Isotricha spp. were without endosymbionts. Polyplastron multivesiculatum invariably had large numbers (> 250 cells per ciliate) of intracellular Bacteria, but no Archaea. Only some Epidinium spp. had intracellular prokaryotes, whereas Entodinium spp. and Ent. simplex almost always had. Many of the larger entodiniomorphid genera were heavily colonized by both bacterial and archaeal species. Eudiplodinium maggii had no obvious bacterial associates, although the autofluorescence of ingested plant material made difficult the detection of possible endosymbionts.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: Dasytricha ruminantium; Polyplastron multivesiculatum; Eudiplodinium maggii; Entodinium simplex; Isotricha intestinalis; Rumen ciliate protozoa; Endosymbionts; In situ hybridization; 16S ribosomal RNA.
Publisher: Elsevier
ISSN: 0932-4739
Last Modified: 03 May 2019 14:09
URI: http://orca.cf.ac.uk/id/eprint/67539

Citation Data

Cited 42 times in Google Scholar. View in Google Scholar

Cited 32 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item