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Regulation of early endosome fusion by phospholipase D activity

Jones, Arwyn Tomos and Clague, Michael J. 1997. Regulation of early endosome fusion by phospholipase D activity. Biochemical and Biophysical Research Communications 236 (2) , pp. 285-288. 10.1006/bbrc.1997.6962

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In vitroor cell-free assays of homotypic fusion between early endosomes have provided several insights into the means by which an intracellular membrane fusion event can be regulated. In this report we show that homotypic fusion between early endosomes from baby-hamster kidney cells is partially blocked by 340mM ethanol (2%v/v) and by 100mM butan-1-ol, but not by the secondary alcohol butan-2-ol. We ascribe the effect of primary alcohols to their participation in a well-characterised transphosphatidylation reaction catalysed by phospholipase D activity, which results in the production of phasphatidylalcohol at the expense of phosphatidic acid. In accordance with this interpretation, we find that addition of exogenous phospholipase D results in stimulation of early endosome fusion.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RS Pharmacy and materia medica
Publisher: Elsevier
ISSN: 0006-291X
Last Modified: 04 Jun 2017 07:45

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