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Glycinergic interneurons are functionally integrated into the inspiratory network of mouse medullary slices

Winter, Stefan M., Fresemann, Jens, Schnell, Christian, Oku, Yoshitaka, Hirrlinger, Johannes and Hülsmann, Swen 2009. Glycinergic interneurons are functionally integrated into the inspiratory network of mouse medullary slices. Pflügers Archiv - European Journal of Physiology 458 (3) , pp. 459-469. 10.1007/s00424-009-0647-1

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Abstract

Neuronal activity in the respiratory network is functionally dependent on inhibitory synaptic transmission. Using two-photon excitation microscopy, we analyzed the integration of glycinergic neurons in the isolated inspiratory pre-Bötzinger complex-driven network of the rhythmic slice preparation. Inspiratory (96%) and ‘tonic’ expiratory neurons (4%) were identified via an increase or decrease, respectively, of the cytosolic free calcium concentration during the inspiratory-related respiratory burst. Furthermore, in BAC-transgenic mice expressing EGFP under the control of the GlyT2-promoter, 50% of calcium-imaged inspiratory neurons were glycinergic. Inspiratory bursting of glycinergic neurons in the slice was confirmed by whole-cell recording. We also found glycinergic neurons that receive phasic inhibition from other glycinergic neurons. Our calcium imaging data show that glycinergic neurons comprise a large population of inspiratory neurons in the pre-Bötzinger complex-driven network of the rhythmic slice preparation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QP Physiology
Publisher: Springer
Last Modified: 12 Jun 2019 03:01
URI: http://orca.cf.ac.uk/id/eprint/67827

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