Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model

Moseley, Ryan, Waddington, Rachel J., Sloan, Alastair James, Smith, Anthony J., Hall, R.C. and Embery, Graham 2003. The influence of fluoride exposure on dentin mineralization using an in vitro organ culture model. Calcified Tissue International 73 (5) , pp. 470-475. 10.1007/s00223-003-0022-8

Full text not available from this repository.

Abstract

This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Dentin ; Mineralization ; Fluoride ; Odontoblasts
ISSN: 14320827
Last Modified: 04 Jun 2017 01:35
URI: http://orca.cf.ac.uk/id/eprint/682

Citation Data

Cited 18 times in Google Scholar. View in Google Scholar

Cited 16 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item