Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library

Lowe, N., Rees, J. S., Roote, J., Ryder, E., Armean, I. M., Johnson, G., Drummond, E., Spriggs, H., Drummond, J., Magbanua, J. P., Naylor, H., Sanson, B., Bastock, R., Huelsmann, S., Trovisco, V., Landgraf, M., Knowles-Barley, S., Armstrong, J. D., White-Cooper, Helen, Hansen, C., Phillips, R. G., Lilley, K. S., Russell, S. and St Johnston, D. 2014. Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library. Development 141 (20) , pp. 3994-4005. 10.1242/dev.111054

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previous P element protein trap screens. Over 20 different research groups then characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Company of Biologists
ISSN: 0950-1991
Date of First Compliant Deposit: 20 February 2019
Date of Acceptance: 13 August 2014
Last Modified: 20 Feb 2019 16:23
URI: http://orca.cf.ac.uk/id/eprint/68320

Citation Data

Cited 5 times in Google Scholar. View in Google Scholar

Cited 45 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 6 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics