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A novel in vitro culture model to investigate the reaction of the dentine-pulp complex to orthodontic force

Dhopatkar, A.A., Sloan, Alastair James, Rock, W.P., Cooper, P.R. and Smith, Anthony J. 2005. A novel in vitro culture model to investigate the reaction of the dentine-pulp complex to orthodontic force. Journal of Orthodontics 32 (2) , pp. 122-132. 10.1179/146531205225020979

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Abstract

Objective: To develop a novel mandible slice organ culture model to investigate the effects of externally applied force on the dentine–pulp complex. Design: In vitro organ culture. Setting: School of Dentistry, Birmingham, UK. Materials and methods: Transverse 2 mm thick sections were cut from the mandibles of five 28-day-old male Wistar rats. Serial sections were used for control and test pairs. Springs made from 0.016-inch and 0.019 x 0.025-inch stainless steel wires were used to apply a 50 g tensile or compressive force, respectively, to test specimens. Control and test specimens were cultured for 5 days in a humidified incubator with 5% CO2 at 37°C and processed for routine histological investigation. Nine more rats were used to provide control and compression test pairs where the pulps were extirpated after 3 days culture and total RNA isolated for gene expression analysis by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Histology showed the dental and supporting tissues maintained a healthy appearance in the control cultures after culture. Histomorphometric analysis revealed a 20–27% increase in pulp fibroblast density in test specimens compared with controls. Gene expression analyses revealed up-regulation in the test groups of PCNA, c-Myc, Collagen 1, TGF-ß1 and alkaline phosphatase, whilst expression of osteocalcin was reduced. Conclusions: The results demonstrated that the present organ culture technique provides a valuable in vitro experimental model for studying the effects of externally applied forces. These forces stimulated a cellular response in the pulp chamber characterized by altered gene expression and proliferation of fibroblasts; the latter being unaffected by the nature of the force in terms of compression or tension.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Organ culture ; orthodontic force ; dentine-pulp complex
Additional Information: "British Orthodontic Society, Chapman Prize Winner 2003"
ISSN: 14653133
Last Modified: 04 Jun 2017 01:35
URI: http://orca.cf.ac.uk/id/eprint/689

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