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ATP inhibition of a mouse brain large-conductance K+ (mslo) channel variant by a mechanism independent of protein phosphorylation

Clark, E. G., Hall, Sarah K. and Shipston, M. J. 1999. ATP inhibition of a mouse brain large-conductance K+ (mslo) channel variant by a mechanism independent of protein phosphorylation. Journal of Physiology 516 (1) , pp. 45-53. 10.1111%2Fj.1469-7793.1999.045aa.x

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Abstract

We investigated the effect of ATP in the regulation of two closely related cloned mouse brain large conductance calcium- and voltage-activated potassium (BK) channel α-subunit variants, expressed in human embryonic kidney (HEK 293) cells, using the excised inside-out configuration of the patch-clamp technique. The mB2 BK channel α-subunit variant expressed alone was potently inhibited by application of ATP to the intracellular surface of the patch with an IC50 of 30 μM. The effect of ATP was largely independent of protein phosphorylation events as the effect of ATP was mimicked by the non-hydrolysable analogue 5′-adenylylimidodiphosphate (AMP-PNP) and the inhibitory effect of ATPγS was reversible. In contrast, under identical conditions, direct nucleotide inhibition was not observed in the closely related mouse brain BK channel α-subunit variant mbr5. Furthermore, direct nucleotide regulation was not observed when mB2 was functionally coupled to regulatory β-subunits. These data suggest that the mB2 α-subunit splice variant could provide a dynamic link between cellular metabolism and cell excitability.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QP Physiology
Publisher: The Physiological Society
Last Modified: 04 Jun 2017 07:53
URI: http://orca.cf.ac.uk/id/eprint/69542

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