Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Quantitative assays for esterified oxylipins generated by immune cells

Morgan, Alwena Haf, Hammond, Victoria Jayne, Morgan, Lloyd, Thomas, Christopher P., Tallman, Keri A., Garcia-Diaz, Yoel Ruslan, McGuigan, Christopher, Serpi, Michaela, Porter, Ned A., Murphy, Robert C. and O'Donnell, Valerie Bridget 2010. Quantitative assays for esterified oxylipins generated by immune cells. Nature Protocols 5 (12) , pp. 1919-1931. 10.1038/nprot.2010.162

Full text not available from this repository.

Abstract

Phospholipid-esterified oxylipins include newly described families of bioactive lipids generated by lipoxygenases in immune cells. Until now, assays for their quantitation were not well developed or widely available. Here, we describe a mass spectrometric protocol that enables accurate measurement of several esterified oxylipins—in particular hydro(pero)xyeicosatetraenoic acids, hydroxyoctadecadienoic acids, hydroxydocosahexaenoic acids and keto-eicosatetraenoic acids—attached to either phosphatidylethanolamine or phosphatidylcholine. Lipids are isolated from cells or tissue using a liquid-phase organic extraction, then analyzed by HPLC–tandem mass spectrometry (LC/MS/MS) in multiple reaction–monitoring mode. The protocol can simultaneously monitor up to 23 species. Generation of standards takes ~2 d. Following this, extraction of 30 samples takes ~3 h, with LC/MS/MS run time of 50 min per sample.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Pharmacy
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RS Pharmacy and materia medica
Uncontrolled Keywords: Biochemistry and protein analysis; isolation, purification and seperation; spectroscopy and structural analysis; synthetic chemistry
Publisher: Nature Publishing Group
ISSN: 1754-2189
Last Modified: 02 Nov 2017 04:12
URI: http://orca.cf.ac.uk/id/eprint/6979

Citation Data

Cited 19 times in Google Scholar. View in Google Scholar

Cited 26 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 14 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item