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A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non-invasive samples

Bidon, Tobias, Frosch, Christiane, Eiken, Hans G., Kutschera, Verena E., Hagen, Snorre B., Aarnes, Siv G., Fain, Steven R., Janke, Axel and Hailer, Frank 2013. A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non-invasive samples. Molecular Ecology Resources 13 (3) , pp. 362-368. 10.1111/1755-0998.12072

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Abstract

We report a new approach for molecular sex identification of extant Ursinae and Tremarctinae bears. Two Y-specific fragments (SMCY and 318.2) and one X-specific fragment (ZFX) are amplified in a multiplex PCR, yielding a double test for male-specific amplification and an internal positive control. The primers were designed and tested to be bear-specific, thereby minimizing the risk of cross-amplification in other species including humans. The high sensitivity and small amplicon sizes (100, 124, 160 base pairs) facilitate analysis of non-invasively obtained DNA material. DNA from tissue and blood as well as from 30 non-invasively collected hair and faeces yielded clear and easily interpretable results. The fragments were detected both by standard gel electrophoresis and automated capillary electrophoresis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QL Zoology
Uncontrolled Keywords: bears; molecular sexing; multiplex PCR; non-invasive; Ursidae ; Ursus arctos
Publisher: Wiley-Blackwell
ISSN: 1755-098X
Last Modified: 04 Jun 2017 07:55
URI: http://orca.cf.ac.uk/id/eprint/69925

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