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Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae)

Hailer, Frank, Gautschi, B. and Helander, B. 2005. Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves : Falconiformes, Accipitridae). Molecular Ecology Notes 5 (4) , pp. 938-940. 10.1111/j.1471-8286.2005.01122.x

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Abstract

We report the development of 14 novel polymorphic microsatellite markers cloned from the White-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has received much conservation attention throughout Eurasia. We also present a protocol for multiplex polymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicilla individuals from southern Sweden, the markers produced two to eight alleles per locus, and average observed and expected heterozygosities were 0.463 and 0.468, respectively. We further present five microsatellite markers that appeared monomorphic in H. albicilla, but which may be of interest for use in other raptor species.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley-Blackwell
ISSN: 1471-8278
Date of Acceptance: 22 June 2005
Last Modified: 04 Jun 2017 07:55
URI: http://orca.cf.ac.uk/id/eprint/69937

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