Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

A molecular analysis of the bacteria present within oral Squamous Cell Carcinoma

Hooper, Samuel James, Crean, St-John Vincent, Fardy, Michael J., Lewis, Michael Alexander Oxenham, Spratt, David A., Wade, William G. and Wilson, Melanie J. 2007. A molecular analysis of the bacteria present within oral Squamous Cell Carcinoma. Journal of Medical Microbiology 56 (12) , pp. 1651-1659. 10.1099/jmm.0.46918-0

Full text not available from this repository.

Abstract

In order to characterize the bacterial microbiota present within oral cancerous lesions, tumorous and non-tumorous mucosal tissue specimens (approx. 1 cm(3)) were harvested from ten oral squamous cell carcinoma (OSCC) patients at the time of surgery. Any microbial contamination on the surface of the specimens was eliminated by immersion in Betadine and washing with PBS. Bacteria were visualized within sections of the OSCC by performing fluorescent in situ hybridization with the universal oligonucleotide probe, EUB338. DNA was extracted from each aseptically macerated tissue specimen using a commercial kit. This was then used as template for PCR with three sets of primers, targeting the 16S rRNA genes of Spirochaetes, Bacteroidetes and the domain Bacteria. PCR products were differentiated by TA cloning and bacterial species were identified by partial sequencing of the 16S rRNA gene fragments. A total of 70 distinct taxa was detected: 52 different phylotypes isolated from the tumorous tissues, and 37 taxa from within the non-tumorous specimens. Differences between the composition of the microbiotas within the tumorous and non-tumorous mucosae were apparent, possibly indicating selective growth of bacteria within carcinoma tissue. Most taxa isolated from within the tumour tissue represented saccharolytic and aciduric species. Whether the presence of these bacteria within the mucosa has any bearing on the carcinogenic process is a concept worthy of further investigation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: Society for General Microbiology
ISSN: 0022-2615
Last Modified: 04 Jun 2017 01:35
URI: http://orca.cf.ac.uk/id/eprint/710

Citation Data

Cited 41 times in Google Scholar. View in Google Scholar

Cited 57 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 24 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item