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A transgenic approach to studying imprinted genes: modified BACs and PACs

John, Rosalind Margaret, Ainscough, Justin and Barton, Sheila C. 2002. A transgenic approach to studying imprinted genes: modified BACs and PACs. In: Ward, Andrew ed. Genomic Imprinting, Vol. 181. Methods in Molecular Biology, Humana Press, pp. 67-81. (10.1385/1-59259-211-2:67)

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Abstract

The advantages of using large genomic clones in the analysis of imprinted genes is described in Chapter 4 with particular reference to yeast artificial chromosomes (YACs). These contain on average 500-600 kb of DNA but can be much larger (>1 Mb). YACS are propagated in yeast and are therefore amenable to genetic modification by homologous recombination, and there are now many examples of their use to generate transgenic mice. This chapter describes a relatively new strategy for using large genomic clones that relies on Escherichia coli-based systems.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH426 Genetics
Publisher: Humana Press
ISBN: 9780896037410
ISSN: 1064-3745
Last Modified: 06 Mar 2020 16:06
URI: http://orca.cf.ac.uk/id/eprint/71191

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