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A simplified method of generating transgenic Xenopus

Sparrow, D. B., Latinkic, Branko and Mohun, T. J. 2000. A simplified method of generating transgenic Xenopus. Nucleic Acids Research 28 (4) , e12. 10.1093/nar/28.4.e12

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Abstract

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Oxford University Press
ISSN: 0305-1048
Last Modified: 04 Jun 2017 08:01
URI: http://orca.cf.ac.uk/id/eprint/71660

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