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Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy

Masia, Francesco, Langbein, Wolfgang Werner, Watson, Peter Duncan and Borri, Paola 2009. Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy. Optics Letters 34 (12) , pp. 1816-1818. 10.1364/OL.34.001816

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Abstract

By detecting the transient four-wave mixing from gold nanoparticles in resonance with their surface plasmon, we demonstrate a multiphoton imaging modality suited for cell microscopy. Four-wave mixing is measured free from background using a three-beam excitation geometry and heterodyne detection. We achieve a spatial resolution of 140 nm in-plane and 470 nm in the axial direction, surpassing the one-photon diffraction limit. With this technique, high-contrast photostable imaging of Golgi structures is demonstrated in HepG2 cells labeled with gold nanoparticles of 10 nm and 5 nm diameter.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Publisher: OSA
ISSN: 0146-9592
Last Modified: 02 May 2019 11:24
URI: http://orca.cf.ac.uk/id/eprint/7258

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