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A reproducible method for the expansion of mouse CD8+ T lymphocytes

Lewis, Mark David, de Leenheer, Evy, Fishman, Sigal, Siew, Lai Khai, Gross, Gideon and Wong, Florence Susan 2015. A reproducible method for the expansion of mouse CD8+ T lymphocytes. Journal of Immunological Methods 417 , pp. 134-138. 10.1016/j.jim.2015.01.004

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Abstract

Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0022-1759
Funders: MRC
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 13 January 2015
Last Modified: 14 Mar 2019 14:07
URI: http://orca.cf.ac.uk/id/eprint/72759

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