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Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells

Lennon, S. V., Ilfeather, S. A. K., Hallett, Maurice Bartlett, Campbell, A. K. and Cotter, T. G. 2008. Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells. Clinical and Experimental Immunology 87 (3) , pp. 465-471. 10.1111/j.1365-2249.1992.tb03021.x

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Previous studies have indicated that Ca2+ is a trigger Tor apoptosis (programmed cell death) in thymocytes and related cell lines. Recently we have shown that levels of apoptosis in leukacmic cells are diminished in Ca2+-deficient conditions, indicating that Ca2+ may be important in the mechanism of apoptosis in these cells. In the present study we investigated the possibility that Ca2+ serves as a trigger for apoptosis in the human leukaemic cell line, HL-60. Using fura-2 to measure cytosolic free Ca2+ concentrations, [Ca2+]i, in cell suspensions, and by using ratio imaging of fura-2 in single cells, we did not observe an early significant increase in [Ca2+]f, in HL-60 cells undergoing apoptosis. The latter stages of apoptosis were, however, accompanied by increasing [Ca2+]f, these increases were apparently a result of, rather than a cause of, apoptosis. Furthermore, apoptosis could be induced in HL-60 cells under conditions of vastly reduced [Ca2+]i achieved by loading these cells with fura-2 in the presence of EGTA. These results indicate that elevation of [Ca2+]i is not a prerequisite for apoptosis in HL-60 cells and that apoptosis can occur in these cells in the presence of low[Ca2+].

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Wiley-Blackwell
ISSN: 0009-9104
Last Modified: 04 Jun 2017 08:07

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