Resistance to and killing by the sporicidal microbicide peracetic acid

Leggett, Mark J., Schwarz, J. Spencer, Burke, Peter A., Mcdonnell, Gerald, Denyer, Stephen P. and Maillard, Jean-Yves ORCID: https://orcid.org/0000-0002-8617-9288 2015. Resistance to and killing by the sporicidal microbicide peracetic acid. Journal of Antimicrobial Chemotherapy 70 (3) , pp. 773-779. 10.1093/jac/dku445

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Abstract

Objectives To elucidate the mechanisms of spore resistance to and killing by the oxidizing microbicide peracetic acid (PAA). Methods Mutants of Bacillus subtilis lacking specific spore structures were used to identify resistance properties in spores and to understand the mechanism of action of PAA. We also assessed the effect of PAA treatment on a number of spore properties including heat tolerance, membrane integrity and germination. Results The spore coat is essential for spore PAA resistance as spores with defective coats were greatly sensitized to PAA treatment. Small acid-soluble spore proteins apparently provide no protection against PAA. Defects in spore germination, specifically in germination via the GerB and GerK but not the GerA germination receptors, as well as leakage of internal components suggest that PAA is active at the spore inner membrane. It is therefore likely that the inner membrane is the major site of PAA's sporicidal activity. Conclusions PAA treatment targets the spore membrane, with some of its activity directed specifically against the GerB and GerK germination receptors.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Publisher:	Oxford University Press
ISSN:	0305-7453
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	13 October 2014
Last Modified:	28 Oct 2022 09:41
URI:	https://orca.cardiff.ac.uk/id/eprint/75341

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