Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens

Sevane Fernandez, Natalia ORCID: https://orcid.org/0000-0003-4766-6291, Bialade, Federica, Velasco, Susana, Rebolé, Almudena, Rodríguez, Maria Luisa, Ortiz, Luís T., Cañón, Javier and Dunner, Susana 2014. Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens. PLoS ONE 9 (6) , e98942. 10.1371/journal.pone.0098942

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Abstract

Inclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥1.4-fold) and 44 down-regulated (≤0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg−1 diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Subjects:	S Agriculture > SF Animal culture
Additional Information:	Copyright: © 2014 Sevane et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher:	Public Library of Science
ISSN:	1932-6203
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	8 May 2014
Last Modified:	05 May 2023 13:52
URI:	https://orca.cardiff.ac.uk/id/eprint/75352

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