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nWASP (neural Wiskott-Aldrich syndrome protein) is a novel therapeutic target in human wound healing [Conference Abstract]

Frugtniet, Bethan, Martin, Tracey Amanda, Harding, Keith Gordon and Jiang, Wen Guo 2014. nWASP (neural Wiskott-Aldrich syndrome protein) is a novel therapeutic target in human wound healing [Conference Abstract]. Wound Repair and Regeneration 22 (5) , A80-A80. 10.1111/wrr.12218

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Abstract

Chronic and nonhealing wounds are a concern for patients and can also represent a significant financial burden on healthcare systems worldwide. Currently, healthcare professionals rely on traditional techniques to treat these difficult wounds, such as wound dressing and compression treatment. This study addresses the challenge of identifying a new therapeutic approach to treat these wounds by the recognition of nWASP as a highly promising target. nWASP has a role in cell motility as a regulator of the actin cytoskeleton and, critically, has been reported as having aberrant activity in human wound tissues. The aims of this study are to examine nWASP levels in human wound tissues and to evaluate the potential of nWASP inhibition in encour- aging wound healing. A cohort of normal, acute, and chronic human wound tissues were collected according to local ethics committee approval. Conventional and quantitative real-time PCR were carried out. Significantly higher levels of nWASP transcripts were found in abnormal/chronic wound tissues compared with normal and acute tissues (p < 0.05). The nWASP inhibitors wiskostatin or 178-1 were applied to HaCaT cells, a human keratinocyte cell line, in the absence or presence of the cell migration inducer HGF. Furthermore, nWASP transcript expression was knocked out by using anti-nWASP transgenes in HaCaT cells. The effect of these processes on cell adhesion and migration following electrical wounding was mea- sured using electrical cell-substrate impedance sensing (ECIS). Statistical analysis of the resulting data was conducted using Minitab, SPSS, and chi-square tests. The healing and migration speed of keratinocytes was increased both by 178-1 and by knockout nWASP (p < 0.05). This observation, combined with the finding of increased nWASP in abnormal/chronic wounds, supports the concept that nWASP inhibition could be an effective treatment for these wounds. Future in vivo studies will be able to further examine the potential of nWASP as a therapeutic target.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: Abstract from the 24th Annual Meeting of the European Tissue Repair Society.
Publisher: Wiley
ISSN: 1067-1927
Last Modified: 21 Mar 2019 21:58
URI: http://orca.cf.ac.uk/id/eprint/75782

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