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Apoptosis in the endothelium of human corneas for transplantation

Albon, Julie, Tullo, Andrew B., Aktar, Saeed and Boulton, Michael E. 2000. Apoptosis in the endothelium of human corneas for transplantation. Investigative Ophthalmology and Visual Science 41 (10) , pp. 2887-2893.

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Abstract

Purpose. To determine whether endothelial cell loss of human corneas stored in organ culture before transplantation is due to apoptosis. Methods. The corneal endothelium of human corneas, stored in organ culture at 34°C for varying periods of time, were analyzed for the presence of apoptotic cells using the TdT-mediated dUTP nick-end labeling (TUNEL) technique. Corneal endothelial cell apoptosis was confirmed by Hoechst staining and immunolabeling with anti–caspase 3 active antibody. Results. Apoptotic cells were identified in the corneal endothelium of human organ cultured corneas: their number and distribution demonstrated a close correlation with corneal folding and overall quality of the corneal endothelium. TUNEL-positive labeling of cells was confirmed as apoptotic by the presence of morphologic nuclear alterations identified by Hoechst staining and the presence of immunostaining for caspase-3 activity. Corneal endothelial cell apoptosis was independent of cause of donor death, death to enucleation time, and death to culture times. Conclusions. Corneal endothelial cell apoptosis appears to determine the suitability of a cornea for transplantation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Publisher: Association for Research in Vision and Ophthalmology
ISSN: 0146-0404
Last Modified: 04 Jun 2017 08:28
URI: http://orca.cf.ac.uk/id/eprint/78861

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