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Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils

Hallett, Maurice, Al-Jumaa, Maha and Dewitt, Sharon 2014. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. In: Quinn, Mark T. and DeLeo, Frank R. eds. Neutrophil Methods and Protocols, Methods in Molecular Biology, vol. 1124. Humana Press, pp. 107-120. (10.1007/978-1-62703-845-4_8)

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Abstract

The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Dentistry
Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Humana Press
ISBN: 978-1-62703-845-4
ISSN: 10643745
Last Modified: 09 Oct 2019 15:00
URI: http://orca.cf.ac.uk/id/eprint/78963

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