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The tumor suppressor folliculin regulates AMPK-dependent metabolic transformation

Yan, Ming, Gingras, Marie-Claude, Dunlop, Elaine, Nouët, Yann, Dupuy, Fanny, Jalali, Zahra, Possik, Elite, Coull, Barry J., Kharitidi, Dmitri, Dydensborg, Anders Bondo, Faubert, Brandon, Kamps, Miriam, Sabourin, Sylvie, Preston, Rachael S., Davies, David, Roughead, Taren, Chotard, Laëtitia, van Steensel, Maurice A.M., Jones, Russell, Tee, Andrew and Pause, Arnim 2014. The tumor suppressor folliculin regulates AMPK-dependent metabolic transformation. The Journal of Clinical Investigation 124 (6) , pp. 2640-2650. 10.1172/JCI71749

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Abstract

The Warburg effect is a tumorigenic metabolic adaptation process characterized by augmented aerobic glycolysis, which enhances cellular bioenergetics. In normal cells, energy homeostasis is controlled by AMPK; however, its role in cancer is not understood, as both AMPK-dependent tumor-promoting and -inhibiting functions were reported. Upon stress, energy levels are maintained by increased mitochondrial biogenesis and glycolysis, controlled by transcriptional coactivator PGC-1α and HIF, respectively. In normoxia, AMPK induces PGC-1α, but how HIF is activated is unclear. Germline mutations in the gene encoding the tumor suppressor folliculin (FLCN) lead to Birt-Hogg-Dubé (BHD) syndrome, which is associated with an increased cancer risk. FLCN was identified as an AMPK binding partner, and we evaluated its role with respect to AMPK-dependent energy functions. We revealed that loss of FLCN constitutively activates AMPK, resulting in PGC-1α–mediated mitochondrial biogenesis and increased ROS production. ROS induced HIF transcriptional activity and drove Warburg metabolic reprogramming, coupling AMPK-dependent mitochondrial biogenesis to HIF-dependent metabolic changes. This reprogramming stimulated cellular bioenergetics and conferred a HIF-dependent tumorigenic advantage in FLCN-negative cancer cells. Moreover, this pathway is conserved in a BHD-derived tumor. These results indicate that FLCN inhibits tumorigenesis by preventing AMPK-dependent HIF activation and the subsequent Warburg metabolic transformation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: PDF uploaded in accordance with publisher's policies at http://www.sherpa.ac.uk/romeo/issn/0021-9738/ (accessed 1.4.16).
Publisher: American Society for Clinical Investigation
ISSN: 0021-9738
Date of First Compliant Deposit: 1 April 2016
Last Modified: 20 Feb 2019 21:30
URI: http://orca.cf.ac.uk/id/eprint/79568

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