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The effect of dentine conditioning agents on solubilisation of bioactive dentine matrix components and dentine regeneration

Sadaghiani, Leili 2015. The effect of dentine conditioning agents on solubilisation of bioactive dentine matrix components and dentine regeneration. PhD Thesis, Cardiff University.
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Abstract

The dentine-pulp complex is a unique, dynamic organ capable of responding to mild and aggressive injurious stimuli. Deep caries or tooth fractures that extend close to the pulp can lead to inflammation and ultimate pulpal necrosis if not managed appropriately. Current therapeutic regimes for such scenarios include the use of vital pulp (capping) treatments, which attempt to stimulate the pulp’s auto-repair capacity which is still poorly understood and consequently not exploited to its full potential. Amongst the possible mechanisms involved in inducing a dentinogenic response in vital pulp therapy, the ability of dental materials in solubilising cell signalling molecules from dentine has been proposed recently. As such, the role of growth factors belonging to TGF-β superfamily in particular has been highlighted during dentine repair and regeneration (Graham et al. 2006). In recent times clinical practice has moved towards the use of dentine bonding agents and composite restorations even in deep dentinal cavities that extend close to the pulp in the hope of reducing the effects of microleakage, amongst others (Lynch 2008). However, although such treatments appear moderately successful in practice, the justifications for introducing new protocols to improve dental pulp protection and dentine bridge formation are not currently driven by biological knowledge. This in vitro study investigated the effects of dentine conditioning by several clinically used etching agents/ dentine conditioners (components of adhesive restorative systems) on the solubilisation of bioactive dentine matrix components and dentine regeneration (by assessing the impact on DPSC phenotype and behaviour). It was found that growth factors (TGF-β1, BMP2 and VEGF) were exposed and released following dentine treatment by EDTA, phosphoric and citric acid. Polyarcrylic acid on the other hand was ineffective in solubilising dentine matrix proteins. Dentine surface conditioning by EDTA, phosphoric and citric acid resulted in an up regulation in the expression of genes associated with osteoblast/odontoblast-like cell differentiation (OPN and ALP) in DPSCs, however surfaces treated with phosphoric acid appeared significantly less supportive of cell growth following 8 days in culture. These findings provide direction for future research and take crucial steps for informing clinical practice as to the choice of materials and techniques in vital pulp treatments.

Item Type: Thesis (PhD)
Status: Unpublished
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Dentine-pulp complex; Dentine repair and regeneration; Dental pulp progenitor cells; Dentine conditioning; Dentine matrix proteins; Vital pulp therapy
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 08:31
URI: http://orca.cf.ac.uk/id/eprint/79592

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