Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Transcriptional consequences of schizophrenia candidate miR-137 manipulation in human neural progenitor cells

Hill, Matthew, Donocik, Jacek G., Nuamah, Rosamond A., Mein, Charles A., Sainz-Fuertes, Ricardo and Bray, Nicholas John 2014. Transcriptional consequences of schizophrenia candidate miR-137 manipulation in human neural progenitor cells. Schizophrenia Research 153 (1-3) , pp. 225-230. 10.1016/j.schres.2014.01.034

[img] PDF - Published Version
Available under License Creative Commons Attribution.

Download (310kB)

Abstract

MIR137, transcribed as the microRNA miR-137, is one of the leading candidate schizophrenia susceptibility genes to arise from large genome-wide association studies (GWAS) of the disorder. Recent data suggest that miR-137 modulates the expression of other schizophrenia susceptibility genes. Although bioinformatic resources are available with which to predict genes regulated by individual microRNA, there has been a lack of empirical data on genome-wide gene expression changes following miR-137 manipulation. We have therefore performed a genome-wide assessment of transcriptional changes in a human neural progenitor cell line after miR-137 over-expression and inhibition in order to elucidate molecular pathways by which genetic perturbation of miR-137 could promote susceptibility to schizophrenia. Bioinformatically-predicted miR-137 targets showed a small but highly significant down-regulation following miR-137 over-expression. Genes that were significantly down-regulated in association with miR-137 over-expression were enriched for involvement in neuronal differentiation. Differentially expressed genes that were confirmed by qPCR included others at genome-wide significant risk loci for schizophrenia (MAD1L1 and DPYD) and BDNF. These data point to molecular pathways through which genetic variation at the MIR137 locus could confer risk for schizophrenia.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Medicine
Neuroscience and Mental Health Research Institute (NMHRI)
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Uncontrolled Keywords: Schizophrenia; GWAS; MicroRNA; miR-137; MIR137; Gene expression; Pathway
Publisher: Elsevier
Funders: MRC
Date of First Compliant Deposit: 30 March 2016
Last Modified: 30 Apr 2019 12:41
URI: http://orca.cf.ac.uk/id/eprint/82231

Citation Data

Cited 28 times in Google Scholar. View in Google Scholar

Cited 47 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics