Imaging ex vivo and in vitro brain morphology in animal models with ultrahigh resolution optical coherence tomography

Bizheva, Kostadinka, Unterhuber, Angelika, Hermann, Boris, Povazay, Boris, Sattmann, Harald, Drexler, Wolfgang, Stingl, Andreas, Le, Tuan, Mei, Michael, Holzwarth, Ronald, Reitsamer, Herbert A., Morgan, John E. and Cowey, Alan 2004. Imaging ex vivo and in vitro brain morphology in animal models with ultrahigh resolution optical coherence tomography. Journal of Biomedical Optics 9 (4) , pp. 719-724. 10.1117/1.1756920

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Abstract

The feasibility of ultrahigh resolution optical coherence tomography (UHR OCT) to image ex vivo and in vitro brain tissue morphology on a scale from single neuron cells to a whole animal brain was investigated using a number of animal models. Sub-2-microm axial resolution OCT in biological tissue was achieved at different central wavelengths by separately interfacing two state-of-the-art broad bandwidth light sources (titanium:sapphire, Ti:Al2O3 laser, lambdac=800 nm, Deltalambda=260 nm, Pout=50 mW and a fiber laser light source, lambdac=1350 nm, Deltalambda=470 nm, Pout=4 mW) to free-space or fiber-based OCT systems, designed for optimal performance in the appropriate wavelength regions. The ability of sub-2-microm axial resolution OCT to visualize intracellular morphology was demonstrated by imaging living ganglion cells in cultures. The feasibility of UHR OCT to image the globular structure of an entire animal brain as well as to resolve fine morphological features at various depths in it was tested by imaging a fixed honeybee brain. Possible degradation of OCT axial resolution with depth in optically dense brain tissue was examined by depositing microspheres through the blood stream to various depths in the brain of a living rabbit. It was determined that in the 1100 to 1600-nm wavelength range, OCT axial resolution was well preserved, even at depths greater than 500 microm, and permitted distinct visualization of microspheres 15 microm in diameter. In addition, the OCT image penetration depth and the scattering properties of gray and white brain matter were evaluated in tissue samples from the visual cortex of a fixed monkey brain.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Society of Photo-optical Instrumentation Engineers (SPIE)
ISSN:	1083-3668
Last Modified:	09 Jan 2018 17:02
URI:	https://orca.cardiff.ac.uk/id/eprint/83529

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