Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Quantitative measurement of fathead minnow vitellogenin mRNA using hybridization protection assays

Thomas-Jones, E, Walkley, Neal, Morris, C., Kille, Peter, Cryer, J., Weeks, I. and Woodhead, J. S. 2003. Quantitative measurement of fathead minnow vitellogenin mRNA using hybridization protection assays. Environmental Toxicology and Chemistry 22 (5) , pp. 992-995. 10.1002/etc.5620220506

Full text not available from this repository.

Abstract

We have developed a novel test system for the quantitative assessment of gene transcription. The procedure involves the use of chemiluminescent-labeled oligonucleotide probes in a hybridization protection assay (HPA) format. We have used this technology to measure changes in vitellogenin mRNA to demonstrate the impact of estrogen exposure in the juvenile fathead minnow (Pimephales promelas). Marked changes in mRNA expression were observed in response to intraperitoneal injection of 17β-estradiol demonstrating the utility of this technique for the identification and monitoring of toxic responses to xenobiotics.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
Publisher: Society of Environmental Toxicology and Chemistry Press
ISSN: 0730-7268
Last Modified: 10 Oct 2017 16:52
URI: http://orca.cf.ac.uk/id/eprint/84515

Citation Data

Cited 11 times in Google Scholar. View in Google Scholar

Actions (repository staff only)

Edit Item Edit Item