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Bridging-type enzyme-linked immunoassay for zinc transporter 8 autoantibody measurements in adult patients with diabetes mellitus

Dunseath, Gareth, Ananieva-Jordanova, Rossitza, Coles, Rebecca, Powell, Michael, Amoroso, Marie, Furmaniak, Jadwiga, Smith, Bernard Rees, Dayan, Colin Mark and Luzio, Stephen 2015. Bridging-type enzyme-linked immunoassay for zinc transporter 8 autoantibody measurements in adult patients with diabetes mellitus. Clinica Chimica Acta 447 , pp. 90-95. 10.1016/j.cca.2015.05.010

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Abstract

Aims A bridging-type ELISA for measuring autoantibodies to zinc transporter 8 (ZnT8A) was assessed using samples from different forms of diabetes mellitus. Methods ZnT8A were measured using an ELISA in patients with type 1 diabetes mellitus (T1DM; n = 94), latent autoimmune diabetes of adulthood (LADA; n = 51), type 2 diabetes mellitus (T2DM; n = 59) and healthy blood donors (HBD; n = 200). ZnT8A in ELISA and immunoprecipitation assays (IPA) using ZnT8 dimer (W325/R325) and monomers (W325, R325 and Q325) were compared. Results Inter- and intra-assay coefficients of variation (CV) were 7.1% and 1.7%, respectively (medium ZnT8A) and 8.5% and 2.7%, respectively (high ZnT8A). In the ELISA 51/94 (54.3%) T1DM, 16/51 (31.4%) LADA and 1/59 (1.7%) T2DM sera were ZnT8A positive. ROC analysis of T1DM and HBD for the ELISA showed 54% sensitivity and 99% specificity (cutoff 15 u/mL) and AUC 0.80 (95% CI, 0.74–0.86). ELISA and IPA measurements were in very good agreement (r = 0.856, k = 0.889, n = 204). Measurement of ZnT8A in addition to autoantibodies for GAD, IA-2 and insulin increased antibody positivity in T1DM by 4.3%, from 80.9% to 85.1%. Conclusions The bridging-type ELISA is a convenient and reproducible method for determination of ZnT8A in serum. Measurement of ZnT8A increased autoantibody positivity in adult T1DM.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: ZnT8A; ELISA; Autoantibody; Type 1 diabetes
Publisher: Elsevier
ISSN: 0009-8981
Date of Acceptance: 15 May 2015
Last Modified: 12 Jun 2019 09:15
URI: http://orca.cf.ac.uk/id/eprint/85359

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