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Interactions of two cytotoxic organotin(IV) compounds with calf thymus DNA

Casini, Angela, Messori, Luigi, Orioli, Pierluigi, Gielen, Marcel, Kemmer, Martine and Willem, Rudolph 2001. Interactions of two cytotoxic organotin(IV) compounds with calf thymus DNA. Journal of Inorganic Biochemistry 85 (4) , pp. 297-300. 10.1016/S0162-0134(01)00215-X

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Abstract

The reactions with DNA of two antitumor active organotin(IV) compounds, the dimer of bis[(di-n-butyl 3,6-dioxaheptanoato)tin] (C52H108Sn4O1·2H2O), compound 1, and tri-n-butyltin 3,6,9-trioxodecanoate (C19H40SnO5·1/2H2O), compound 2, were analysed by circular dichroism, DNA melting experiments and gel mobility shift assays. It is found that both complexes modify only slightly the B-type circular dichroism spectroscopy (CD) spectrum of calf thymus DNA. On the other hand, both complexes were found to affect significantly the parameters of the thermally induced helix-to-coil transition. Addition of 1 or 2 to calf thymus DNA samples does not favor DNA renaturation after melting ruling out formation of interstrand crosslinks. Moreover, the effects of both compounds on plasmid DNA gel mobility were investigated. From the analysis of the present results it is inferred that both organotin(IV) compounds do interact with DNA, probably at the level of the phosphate groups.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Uncontrolled Keywords: Organotin compounds; DNA; Circular dichroism; DNA melting; Gel electrophoresis
Publisher: Elsevier
ISSN: 0162-0134
Date of Acceptance: 21 March 2001
Last Modified: 04 Jun 2017 08:49
URI: http://orca.cf.ac.uk/id/eprint/85560

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