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Effects of two representative antitumor ruthenium(III) complexes on thermal denaturation profiles of DNA

Messori, L, Casini, Angela, Vullo, D, Haroutiunian, S.G, Dalian, E.B and Orioli, P 2000. Effects of two representative antitumor ruthenium(III) complexes on thermal denaturation profiles of DNA. Inorganica Chimica Acta 303 (2) , pp. 283-286. 10.1016/S0020-1693(99)00528-9

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Abstract

The effects of two representative antitumor ruthenium(III) complexes — namely (dichloro-1,2-propylenediamine tetraacetate ruthenium(III)) Ru(III)) RAP and (sodium trans-(dimethylsulfoxide)(imidazole)tetrachloro ruthenate(III)) NAMI — on thermal denaturation profiles of calf thymus DNA were analyzed. It is shown that both complexes slightly stabilize calf thymus DNA when tested in the concentration range 0.01<r<0.1; minor effects on the melting interval ΔT are also observed. Differential melting curves permit monitoring of the effects of ruthenium complexes, on the different regions of calf thymus DNA depending on their GC content. Preferential binding of NAMI to GC-rich tracts of DNA is observed. Notably both complexes favor renaturation of calf thymus DNA although to a lesser extent than cisplatin does. CD spectra reveal only very modest conformational changes of DNA upon addition of either ruthenium complex in the concentration range used in the melting studies. Implications of the present results for the mechanism of action of antitumor ruthenium(III) complexes are discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Uncontrolled Keywords: Antitumor activity; Ruthenium complexes
Publisher: Elsevier
ISSN: 0020-1693
Date of Acceptance: 8 November 1999
Last Modified: 04 Jun 2017 08:49
URI: http://orca.cf.ac.uk/id/eprint/85561

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