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DNA as a possible target for antitumor ruthenium(111) complexes - A spectroscopic and molecular biology study of the interactions of two representative antineoplastic ruthenium(111) complexes with DNA

Gallori, Enzo, Vettori, Cristina, Alessio, Enzo, Vilchez, Francisco Gonzalez, Vilaplana, Rosario, Orioli, Pierluigi, Casini, Angela and Messori, Luigi 2000. DNA as a possible target for antitumor ruthenium(111) complexes - A spectroscopic and molecular biology study of the interactions of two representative antineoplastic ruthenium(111) complexes with DNA. Archives of biochemistry and biophysics 376 (1) , pp. 156-162. 10.1006/abbi.1999.1654

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Abstract

The interaction of two experimental ruthenium(III)-containing antitumor complexes—Na[trans-RuCl4(DMSO)(Im)] (NAMI) and dichloro(1,2-propylendiaminetetraacetate)ruthenium(III) (RAP)—with DNA was investigated through a number of spectroscopic and molecular biology techniques, including spectrophotometry, circular dichroism, gel shift analysis, and restriction enzyme inhibition. It was found that both complexes slightly alter DNA conformation, modify its electrophoretic mobility, and inhibit DNA recognition and cleavage by some restriction enzymes, though they were less effective than cisplatin in producing such effects. Notably, the effects produced by NAMI on DNA were much larger than those induced by RAP. Implications of these results for the mechanism of action of ruthenium(III) antitumor complexes are discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Uncontrolled Keywords: ruthenium complexes; cancer; DNA; circular dichroism; restriction enzymes; electrophoresis
Publisher: Elsevier
ISSN: 0003-9861
Last Modified: 04 Jun 2017 08:49
URI: http://orca.cf.ac.uk/id/eprint/85562

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