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Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity

Morris, Laura S. and Marchesi, Julian Roberto 2016. Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity. Journal of Microbiological Methods 123 , pp. 31-38. 10.1016/j.mimet.2016.02.001

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Abstract

The proteome is the second axis of the microbiome:host interactome and proteases are a significant aspect in this interaction. They interact with a large variety of host proteins and structures and in many situations are implicated in pathogenesis. Furthermore faecal samples are commonly collected and stored frozen so they can be analysed at a later date. So we were interested to know whether long term storage affected the integrity of proteases and total protein and whether historical native faecal samples were still a viable option for answering research questions around the functional proteome.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: Gut; Microbiota; Proteases; Proteins; Long-term storage; Enzyme activity
Publisher: Elsevier
ISSN: 0167-7012
Funders: Biotechnology and Biological Sciences Research Council
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 3 July 2015
Last Modified: 04 Jun 2017 08:53
URI: http://orca.cf.ac.uk/id/eprint/87076

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