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Actin and type I collagen propeptide distribution in the developing chick cornea

Gealy, Elizabeth Claire, Hayes, Anthony Joseph, Buckwell, Rebecca, Young, Robert David, Caterson, Bruce, Quantock, Andrew James and Ralphs, James Robert 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50 (4) , 1653 -1658. 10.1167/iovs.08-2554

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Abstract

purpose. To determine the organization of actin filaments and distribution of type I procollagen during the development of the chick corneal stroma. methods. Embryonic chicken corneas of ages 6 to 18 days and 18 days posthatch were cryosectioned and fluorescently labeled for filamentous actin with phalloidin and for the N-and C-terminal propeptides of type I procollagen with specific monoclonal antibodies. Tissue sections were examined by fluorescence and confocal microscopy. results. Prominent actin filament bundles were present at all embryonic stages, arranged in orthogonal arrays. Type I collagen propeptides were also present, with the C-propeptide visible as small foci, often associated with the actin label. The N-propeptide was also detected in the stromal matrix, especially in Bowman’s layer. Actin filaments were also prominent in the corneal epithelium, along with collagen propeptide labeling, up to embryonic day14. conclusions. Actin filament bundles are abundant in the stroma, presumably in the keratocytes of the developing chick cornea, and are arranged in an orthogonal manner suggesting a possible role in cell and matrix organization in this tissue. Filament bundles appear to be closely associated with the foci of type I procollagen label, suggesting a possible association between the actin cytoskeleton and the trafficking of collagen. The presence of the N-propeptide of type I collagen in the extracellular matrix and the restricted distribution of the C-propeptide suggest differential processing of these molecules after secretion. The persistence of the N-propeptide implies a role in development, possibly in association with control of collagen fibril diameter and spacing.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Optometry and Vision Sciences
Subjects: Q Science > QH Natural history
Q Science > QR Microbiology
Additional Information: Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher: Association for Research in Vision and Ophthalmology
ISSN: 0146-0404
Date of First Compliant Deposit: 30 March 2016
Last Modified: 03 May 2019 22:59
URI: http://orca.cf.ac.uk/id/eprint/8724

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