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Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease

Bascoul-Colombo, Cécile, Guschina, Irina, Maskrey, Benjamin H., Good, Mark Andrew, O'Donnell, Valerie Bridget and Harwood, John L. 2016. Dietary DHA supplementation causes selective changes in phospholipids from different brain regions in both wild type mice and the Tg2576 mouse model of Alzheimer's disease. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1861 , pp. 524-537. 10.1016/j.bbalip.2016.03.005

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Abstract

Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids and amyloid pathology. Because dietary docosahexaenoic acid(DHA) has been identified as beneficial, we compared mice fed with a DHA-supplemented diet to those on a nutritionally-sufficient diet.Major phospholipids from cortex, hippocampus and cerebellum were separated and analysed. Each phosphoglyceride had a characteristic fatty acid composition which was similar in cortex and hippocampus but different in the cerebellum. The biggest changes on DHA-supplementation were within ethanolamine phospholipids which, together with phosphatidylserine, had the highest proportions of DHA. Reciprocal alterations in DHA and arachidonate were found. The main diet-induced alterations were found in ethanolamine phospholipids, (and included their ether derivatives), as were the changes observed due to genotype. Tg mice appeared more sensitive to diet with generally lower DHA percentages when on the standard diet and higher relative proportions of DHA when the diet was supplemented. All four major phosphoglycerides analysed showed age-dependent decreases in polyunsaturated fatty acid contents. These data provide, for the first time, a detailed evaluation of phospholipids in different brain areas previously shown to be relevant to behaviour in the Tg2576 mouse model for AD. The lipid changes observed with genotype are consistentwith the subtle alterations found in AD patients, especially for the ethanolamine phospholipidmolecular species. They also emphasise the contrasting changes in fatty acid content induced by DHA supplementation within individual phospholipid classes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Psychology
Additional Information: This journal has an embargo period of 12 months. PDF uploaded in accordance with publisher's policies at http://www.sherpa.ac.uk/romeo/issn/1388-1981/ (accessed 10.3.16).
Publisher: Elsevier
ISSN: 1388-1981
Funders: Wellcome Trust
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 4 March 2016
Last Modified: 28 May 2018 13:29
URI: http://orca.cf.ac.uk/id/eprint/87704

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