Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Targeting RhoC by way of ribozyme trangene in human breast cancer cells and its impact on cancer invasion

Lane, Jane, Martin, Tracey Amanda and Jiang, Wen Guo 2010. Targeting RhoC by way of ribozyme trangene in human breast cancer cells and its impact on cancer invasion. World Journal of Oncology 1 (1) , pp. 7-13. 10.4021/wjon2010.01.1202

Full text not available from this repository.


Background: Cell motility and migration are known to be regulated by the Rho family of GTPases through their effects on the actin cytoskeleton. In breast cancer studies, RhoC has been identified as a highly specific marker in detecting tumors that developed metastases. This study aims to investigate the impact of targeting RhoC in human breast cancer cells by utilising ribozyme transgene technology and to assess its effect on cancer cell invasion. Methods: Retroviral hammerhead ribozyme transgenes, regulated by doxycycline, were designed to specifically target human RhoC mRNA. The breast cancer cell line MDA-MB-231 was transfected with either a retroviral RhoC transgene or a control retroviral transgene. Stably transfected cells were tested for their invasiveness and migratory properties in vitro. Results: In vitro testing of the invasiveness of wild type, plasmid control and the RhoC knockdown cells showed that MDA-MB-231DRHOC cells had significantly reduced invasiveness compared with MDA-MB-231WT (p < 0.038 RHOC2 knockdown cells; p < 0.006 RHOC3 knockdown cells) and MDA-MB-231pRevTRE control plasmid cells (p < 0.07 RHOC2 knockdown cells; p < 0.002 RHOC3 knockdown cells). An even greater reduction in invasiveness of the MDA-MB-231DRHOC cells compared with the MDA-MB-231WT cells was seen in response to hepatocyte growth factor (HGF/SF) (p < 0.009 RHOC1 knockdown; p = 0.004 RHOC2 knockdown; p = 0.00007 RHOC3 knockdown). The addition of doxycycline significantly improved the effectiveness of the ribozyme transgenes (p < 0.04 for all three Rho ribozymes), but did not improve the effectiveness of these knockdown cells when treated with HGF/SF (p > 0.1 for all three ribozymes). Conclusions: This data would indicate that targeting RhoC may be an effective way to reduce the invasive potential of human breast cancer cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: RhoC; Invasion; Ribozyme; Molecular targeting; Breast cancer
Publisher: Elmer Press
ISSN: 1920-4531
Funders: Cancer Research Wales, Breast Cancer Hope Foundation
Date of Acceptance: 8 January 2010
Last Modified: 23 Apr 2019 21:27

Actions (repository staff only)

Edit Item Edit Item