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Wrestling with the repertoire: the promise and perils of next generation sequencing for antigen receptors

Baum, Paul D., Venturi, Vanessa and Price, David 2012. Wrestling with the repertoire: the promise and perils of next generation sequencing for antigen receptors. European Journal of Immunology 42 (11) , pp. 2834-2839. 10.1002/eji.201242999

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Abstract

Next generation sequencing technologies are revolutionizing the study of immune repertoires. These methods provide a previously unimaginable amount of sequence data, unfortunately accompanied by numerous challenges associated with error correction and interpretation that remain to be solved. For antigen receptors, these challenges will require dedicated solutions beyond those developed for genome sequencing, which may differ depending on the sequencing technology used and the purpose of the experiment. Many investigators are developing such methods, based on different sequencing platforms, but critical details of protocol and performance are proprietary. The field will move forward when these methods are shared and standardized, and when the accuracy, sensitivity and reproducibility of various sequencing, and analytic methods are evaluated using standardized samples in comparative experiments.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QR Microbiology > QR180 Immunology
Publisher: John Wiley & Sons
ISSN: 0014-2980
Date of First Compliant Deposit: 4 April 2016
Date of Acceptance: 1 October 2012
Last Modified: 04 Jun 2017 08:58
URI: http://orca.cf.ac.uk/id/eprint/88593

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