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Mast cells produce a unique chondroitin sulfate epitope

Farrugia, B. L., Whitelock, J. M., O'Grady, R., Caterson, Bruce and Lord, M. S. 2016. Mast cells produce a unique chondroitin sulfate epitope. Journal of Histochemistry and Cytochemistry 64 (2) , pp. 85-98. 10.1369/0022155415620649

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Abstract

The granules of mast cells contain a myriad of mediators that are stored and protected by the sulfated glycosaminoglycan (GAG) chains that decorate proteoglycans. Whereas heparin is the GAG predominantly associated with mast cells, mast cell proteoglycans are also decorated with heparan sulfate and chondroitin sulfate (CS). This study investigated a unique CS structure produced by mast cells that was detected with the antibody clone 2B6 in the absence of chondroitinase ABC digestion. Mast cells in rodent tissue sections were characterized using toluidine blue, Leder stain and the presence of mast cell tryptase. The novel CS epitope was identified in rodent tissue sections and localized to cells that were morphologically similar to cells chemically identified as mast cells. The rodent mast cell-like line RBL-2H3 was also shown to express the novel CS epitope. This epitope co-localized with multiple CS proteoglycans in both rodent tissue and RBL-2H3 cultured cells. These findings suggest that the novel CS epitope that decorates mast cell proteoglycans may play a role in the way these chains are structured in mast cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: The Histochemical Society
ISSN: 0022-1554
Date of First Compliant Deposit: 7 April 2016
Date of Acceptance: 11 November 2015
Last Modified: 18 Oct 2019 22:42
URI: http://orca.cf.ac.uk/id/eprint/88739

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